Enzyme-linked immunosorbent assay is based on immunology. It can detect thousands of biological macromolecular targets such as Albumin, IgA, IgD, IgM, etc. It can detect pesticides, antibiotics, metabolites and other small molecular targets. Affinity determination can also be performed, such as determining the affinity of an antigen and its specific antibody. It can even be used to detect pesticide residues and toxin content in various foods. Creative Diagnostics has a strong product library, and we provide ELISA testing services for each type of product. And our technicians have been engaged in ELISA testing for a long time and have rich experience, we can provide the most accurate test results.
Enzyme-linked immunosorbent assay is based on immunology. First, the antigen or antibody is immobilized, and the antigen or antibody is labeled with an enzyme. The solid-phase antigen or antibody retains its immunological activity, while the enzyme-labeled antigen or antibody retains its immunological activity and enzymatic activity. In ELISA detection, the antigen or antibody in the sample is combined with the solid phase antigen or antibody, the excess free reactant is removed by washing, and then the substrate reacted with the enzyme is added for color development, and qualitative or quantitative analysis is performed according to the color depth.
Creative Diagnostics has a huge number of product libraries, and our technicians have long been engaged in ELISA testing and have rich experience. After receiving your samples, we will strictly abide by industry regulations, use our comprehensive products, advanced instruments, and high-standard laboratories to return to you reliable and stable test results as soon as possible. We provide customers with ELISA testing services and analysis of test results including dilution linearity and spike recovery analysis.
In practical applications, we will choose the most suitable ELISA detection method for you according to your sample type, detection target, and research purpose. Through different designs, there are many specific method steps. For example, the indirect method used to detect antibodies, the double antibody sandwich method used to detect antigens, and the antigen competition method used to detect small molecule antigens or haptens.
1 If the indirect method is used to detect antibodies, the customer must provide sufficient primary antibodies (or buy them by Creative Diagnostics), and please provide detailed instructions for commercial antibodies so that we can perform accurate experiments by the instructions.
2 If the sandwich method is used to detect antibodies, the customer must provide sufficient antigen. The purity and quality requirements of the antigen will be determined after a discussion based on the experimental requirements.
3 Customers do not need to provide secondary antibodies. To ensure the smoothness and accuracy of the test, the antibody and antigen information provided by the customer must be accurate and detailed. Our researchers will operate following Elisa's standard SOP process.
Creative Diagnostics provides not only the most accurate and reproducible ELISA testing services but also comprehensive ELISA development services and ELISA kit customization services. If you are interested in our services, please contact us for details.