Indirect ELISA has the advantage of being highly sensitive, more economical, and more flexible. The use of secondary antibodies in indirect ELISA allows the detection signal to be amplified many times, but it is also the use of secondary antibodies that leads to the possibility of cross-reactivity (direct binding of the enzyme-labeled secondary antibody to the antigen), which may increase the background.
Creative Diagnostics offers hundreds of indirect ELISA kits for antibodies against a wide range of anti-pathogens and food allergens, as well as food allergens, and our products cover more than a dozen species reactivity in human, mouse, rat, rabbit, etc. Please click the button below to enter the product list page.