Sandwich ELISA is a type with very high sensitivity and specificity, attributed to the need to use paired antibodies (capture antibody and detection antibody). Sandwich ELISAs are particularly suitable for the detection of large molecular antigens or antibodies. If the detection antibody is enzyme-labeled, it is referred to as a direct sandwich ELISA; if the detection antibody is unlabeled, it is also necessary to use an enzyme-labeled secondary antibody to bind to the detection antibody, which is referred to as an indirect sandwich ELISA.

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