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Arsenic Contaminants

The Severity of Environmental Contamination Caused by Arsenic

Arsenic (As) is a kind of metalloid, arsenic contaminants mainly come from the mining and smelting of arsenic and arsenic-containing metals, the production of glass, pigments, technical materials, paper, and the burning of coal using arsenic or arsenic compounds as raw materials. Arsenic-containing wastewater, waste gas, and waste residues enter the environment and pollute the atmosphere, water bodies, and soil. Arsenic is very toxic to aquatic organisms and can accumulate in the soil and enter the crop tissues. An arsenic poisoning incident in Bangladesh in 2009 may have caused a collective arsenic poisoning of two million people and caused many deaths. On December 15, 2004, the World Health Organization officials announced that at least more than 50 million people in the world are facing the threat of endemic arsenic poisoning. Arsenic pollution is approaching people to step by step, seriously threatening people's health and life safety.

The Necessity of Monitoring Arsenic Contamination with ELISA Testing

Many compounds of arsenic contain lethal toxicity, and trivalent arsenic is the most toxic. Arsenic and arsenic compounds generally enter the human body through inhalation of arsenic-containing air or ingestion of arsenic-contaminated food and drinking water, causing poisoning incidents. Arsenic can be combined with sulfhydryl-containing enzymes in cells to inhibit the cell oxidation process, and can also paralyze the vascular movement center, paralyze, dilate and increase the permeability of capillaries. The International Agency for Research on Cancer of the World Health Organization has listed arsenic and inorganic arsenic compounds as a class of carcinogens, and arsenic and arsenic compounds have also been included in the list of toxic and harmful water pollutants. Faced with such a severe arsenic pollution situation, it is necessary to monitor the concentration of arsenic in the environment. As a detection method with high sensitivity, good specificity, fast and simple, ELISA is widely used in the detection of arsenic concentration.

ELISA Type

Indirect Competitive ELISA

The Advantages of ELISA Testing

High sensitivity and low cost
High-throughput detection and analysis
No complicated sample preparation and purification
High recovery rate, small coefficient of variation
Can be used for the determination of organoarsenic in animal tissues and feed

ELISA Procedure for Heavy Metal Contaminants organoarsenic Testing

The microplates were coated with coating conjugates in 100 μL of coating buffer (CBS, 0.05 mol/L, pH 9.6) overnight at 4°C.

The plates were washed three times with PBS containing 0.1% Tween-20 and were incubated with 200 μL of 1% OVA in PBS for 0.5 h at 37°C.

After washing with PBST, the antiserum (100 μL/well) was added to the plates and incubated for 30 min at 37°C.

The washed plates with PBST were added with 100 μL of HRP-IgG (1:5000) and incubated for 30 min at 37°C.

Then, the washed plate was added with 100 μL/well TMB substrate solution, incubated for 15 min at room temperature in the dark.

Terminated by adding 2M H₂SO₄ and the absorbance measured at 450 nm using a microplate reader.

Creative Diagnostics has been committed to heavy metal contaminants testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of organoarsenic. If you wish a lot of careful data, please contact us.

References

Datta, R.; et al. Arsenic biogeochemistry and human health risk assessment in organo-arsenical pesticide-applied acidic and alkaline soils: an incubation study. Sci Total Environ. 2006, 372(1): 39-48.
Zhu, X.D.; et al. Kinetics, intermediates and acute toxicity of arsanilic acid photolysis. Chemosphere. 2014, 107: 274-281.
Peng, D.; et al. Development and validation of an indirect competitive enzyme-linked immunosorbent assay for monitoring organoarsenic compounds in edible chicken and pork and feed. Food Chem. 2016, 197(Pt A): 821-828.

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