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Chromium Contaminants

The Severity of Environmental Contamination Caused by Chromium

Chromium (Cr) contaminants mainly come from chromium mining, smelting, chromium salt manufacturing, electroplating, metal processing, tanning, paint, pigments, printing and dyeing industries, and chromium-containing waste gas, wastewater, and waste residues discharged from fuel combustion. Chromium is a trace element necessary for humans and animals, the lack of chromium in the body can cause atherosclerosis. Chromium has a stimulating effect on plant growth and can increase the yield. However, excessive chromium can cause harmful effects on humans, animals, and plants. Chromium and its compounds are highly toxic to the human body and can accumulate in the human body. Chromium contaminants have become an important problem of environmental pollution, which needs to be solved urgently.

The Necessity of Monitoring Chromium Contamination with ELISA Testing

The valences of chromium are divalent, trivalent, and hexavalent. Trivalent chromium and hexavalent chromium in the environment can be transformed into each other. Hexavalent chromium is about 100 times more toxic than trivalent chromium and is a strong mutagenic substance that can induce lung cancer and nasopharyngeal cancer, and trivalent chromium has teratogenic effects. Ingestion of chromium-contaminated food or water can cause abdominal discomfort, diarrhea, and other poisoning symptoms, causing allergic dermatitis or eczema; breathing in chromium-contaminated gas has irritation and corrosive effects on the respiratory tract, causing pharyngitis, bronchitis, etc. Given the strong toxicity of chromium, there are strict index control standards for pollutant discharge. Therefore, it is very important to monitor the concentration of chromium in environmental samples. As a detection method with high sensitivity, good specificity, fast, simple, and accurate, ELISA is used in the detection of chromium.

ELISA Type

Sandwich ELISA

The Advantages of ELISA Testing

High accuracy
Enhanced detection signal
Increased binding of capture antibody
High sensitivity and specificity to chromium
High-throughput detection and analysis of chromium

ELISA Procedure for Heavy Metal Ions Chromium (III) Testing

The microplates were coated with the capture antibodies (50 μL/well) which were diluted in TBS (150mM NaCl, pH 8.0) and incubated for 2 h at room temperature.

The microplates were washed three times with TBS+0.05% Tween 20 (350 μL) and blocked with 1% BSA diluted in TBS (100 μL) and incubated for 1 h at room temperature on an orbital shaker.

After washing, the antigen (50 μL/well) was prepared in TBS was added and incubated for 1 h at room temperature on an orbital shaker.

Then the HRP tagged detection antibody (1 μg/mL) in TBS was applied (50 μL/well) and incubated for 30 min at room temperature on an orbital shaker.

Empty wells were used to prepare a control of the relative signal intensity. After washing, 25 ng of anti-FLAG-HRP was added, then the substrate was added to the entire plate.

The SuperSignal ELISA Pico Chemiluminescent Substrate diluted in TBS by 1:1:6 was added to the plate (overall 1/4 prescribed strength), 50 μL/well.

KPL SureBlue TMB Microwell Peroxidase Substrate was added (50 μL/well) and incubated at room temperature for approximately 5-10 min.

Terminated by adding 50 μL 0.5M HCl, and the absorbance was measured at 460 nm in the microplate reader.

Creative Diagnostics has been committed to heavy metal contaminants testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of chromium. If you wish a lot of careful data, please contact us.

References

McCarron, P.; et al. Self reported health of people in an area contaminated by chromium waste: interview study. BMJ. 2000, 320(7226): 11-15.
Welch, N.G.; et al. A chemiluminescent sandwich ELISA enhancement method using a chromium (III) coordination complex. J Immunol Methods. 2016, 438: 59-66.

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