Development of Fiber-Based ELISA
Fiber-based ELISA is similar to paper-based ELISA in that a new ELISA format is established by applying new materials to it following the principle of conventional ELISA. Creative Diagnostics has many years of ELISA development experience, not only providing you with a large number of ELISA kits, but also providing you with fiber-based ELISA development services that meet your specific needs and regulatory requirements.
Why Choose Development of Fiber-Based ELISA?
Fiber-based ELISA and paper-based ELISA have similarities but also differences. The potential of fiber-based technology platforms is enormous. Fibers have high volume/mass transfer rates, tunable chemistry, non-denaturing, and regenerative surfaces. In particular, fiber-based ELISA can be enhanced by changing the surface properties of the fibers. if you want to develop novel ELISA forms with these characteristics, you may consider choosing our fiber-based ELISA development services.
Questions to Consider Before Choosing This Service
- Do you need to develop a fiber-based immunoassay platform?
- Do you need to develop a flexible, high-strength detection platform that can be used for cumbersome and lengthy detection?
If your answer is yes, then congratulations that you can choose us to develop a fiber-based ELISA for you. Alternatively, you can tell us all your thoughts and we will discuss together and then choose the ELISA format you need.
Advantages and Disadvantages of Fiber-Based ELISA
In fiber-based ELISA, fibers with better physical properties such as hydrophobicity, porosity, and surface area as well as chemical properties are introduced as solid-phase carriers for targets detection strategies.
The Main Advantages of Fiber-Based ELISA
- Longer shelf life of fiber material.
- Overcomes the lack of sensitivity of paper-based ELISA
- Customized fiber material performs better than the cellulose membrane in paper-based ELISA.
- Ability to introduce more reactive -COOH functional groups on the surface of the fiber material.
The Main Disadvantage of Fiber-Based ELISA
- Repeatability may be poor.
Fig. 1 The schematic illustration of fiber-based ELISA for dengue. (Elham Farahmand, et al., 2015)
Development of Fiber-Based ELISA
In our fiber-based ELISA development services, we follow the gold standard protocol while prioritizing high sensitivity, stability, and customized physical and chemical properties of the material, and strictly follow the workflow below to provide you with a trustworthy fiber-based ELISA development solution.
We will design a fiber-based ELISA development strategy based on your target and taking into account multiple considerations.
As with conventional ELISA development, we will design and synthesize the gene and prepare the antigen-based on your target.
With years of experience in antibody customization, we will prepare high-quality antibodies with high purity and concentration to meet your needs.
In this step, we will use the most advanced technology to determine the affinity of antibodies.
We will select the most suitable enzyme-labeled secondary antibody for you based on the characteristics of the primary antibody.
In fiber-based ELISA, the fiber material needs to be processed, so we need to select various reagents such as methyl methacrylate (MMA), N-hydroxysuccinimide (NHS), bovine serum albumin (BSA), and tetrahydrofuran (THF).
Before coating the fiber membrane, the polymer needs to be synthesized. Typically, several different poly (MMA-co-MAA) compositions are polymerized via free radical polymerization reactions and freeze-dried for storage.
The coating of the fiber membrane is one of the key points in the development of fiber-based ELISA. We prepared the polymer synthesized in the previous step as a solution and coated the fiber membrane.
After coating the fiber membrane, we need to analyze the morphology of the fiber membrane using atomic force microscopy (AFM) and scanning electron microscopy (SEM), and we need to analyze the polymer by Fourier transform infrared spectroscopy (FTIR). After ensuring that there are no errors, proceed to the next step of the process.
We will use the prepared fiber membrane for the development of the fiber-based ELISA. Typically, the fiber-based ELISA is based on innovation on the sandwich ELISA.
Combining various assay numbers, we need to optimize all aspects involved in fiber-based ELISA.
In the validation step, we routinely perform a fiber-based ELISA assay on your specified biological sample to ensure that the recombinant protein/peptide assay is consistent with the target.
Once the development protocol has been validated, we will produce the specified fiber-based ELISA kit according to your requirements and provide all the reagents and materials involved in the protocol.
Result/Material Delivery
Development Protocol
All Experi-mental Data
Ready-to-use ELISA Kits
Validated Assay Results
Creative Diagnostics makes every effort to provide you with a fiber-based ELISA development service to meet any of your needs. We also offer appropriate ELISA testing services, as well as comprehensive solutions for your ELISA kit customization needs. If you have a need, please contact us directly.
Reference
- Elham Farahmand, et al. (2015). "A novel approach for application of nylon membranes in the biosensing domain." Applied Surface Science. 353, 1310-1319.
