Development of Microfluidic ELISA
ELISA is a powerful immunoassay technique for the rapid and specific quantitative detection of proteins, peptides, hormones, etc. in various biological samples. However, it is mostly limited to the type of samples and has a very limited variety of enzymes that can be used. To overcome these limitations, microfluidic devices were introduced into ELISA and microfluidic ELISA was established. Creative Diagnostics, as a cutting-edge ELISA developer, can provide you with microfluidic ELISA development services based on your needs.
Why Choose Development of Microfluidic ELISA?
The microfluidic ELISA detection technology subverts the conventional ELISA, and innovatively coats the capture antibody in the microfluidic device. Sample separation, antigen-antibody coupling, washing, substrate addition, etc. are all carried out in the microfluidic device, which greatly reduces the error caused by the human operation. And the number of detectable samples increases, which greatly increases the application value of ELISA. If you want to develop new ELISA technology with these characteristics, then you can choose our microfluidic ELISA development service.
Questions to Consider Before Choosing This Service
- Do you need to develop a portable chip system that can perform ELISA detection on multiple samples and multiple targets simultaneously?
- Do you need to develop an ELISA format that can detect a wider range of samples?
- Do you need to develop ELISA assays that can be used under limited conditions?
If your answer is yes, then basically this ELISA development service can meet your needs. If you are not yet sure, we will provide you with scientific consultation to choose the best format for ELISA development for you.
Advantages and Disadvantages of Microfluidics ELISA
Microfluidic ELISA is an ELISA type with higher sensitivity and a wider dynamic range that introduces microfluidic equipment and can perform high-throughput detection of multiple samples at the same time.
The Main Advantages of Microfluidic ELISA
- Detection takes less time.
- Only a smaller sample size is required.
- Increased sensitivity and selectivity.
- Ability to detect multiple samples simultaneously.
- Reduced human contact with hazardous materials.
The Main Disadvantage of Microfluidic ELISA
- Requires more complex components.
Fig. 1 The schematic illustration of microfluidic ELISA. (Lee, B., et al., 2009)
Development of Microfluidic ELISA
In addition to conventional services, the development of microfluidic ELISA also involves the synthesis of ELISA substrates, the manufacture of microfluidic devices, and the preparation of ELISA surfaces. To provide you with a perfect automated microfluidic ELISA development program, we will strictly follow the development process below.
We will develop a development strategy based on your detection target and your various requirements for the microfluidic ELISA (such as detection limit, sensitivity, specificity, etc.).
We have rich experience in antigen preparation and will prepare the corresponding antigen according to your target (single or multiple targets) to be tested.
Based on the prepared antigen, we will use a variety of antibody development systems to prepare the highest standard antibody for you.
The affinity of the antibody was assayed and analyzed according to routine operations.
In the development of microfluidic ELISAs, we typically use alkaline phosphatase to label antibodies.
In the development of microfluidic ELISA, we usually choose to develop a rhodamine-based ELISA substrate to better bind and develop color with the secondary antibody labeled with alkaline phosphatase.
Fabrication of microfluidic devices is a crucial step in the development of microfluidic ELISAs. We will manufacture the corresponding microfluidic devices according to the development strategy, most commonly CD-shaped microfluidic devices made of glass and PDMS.
The prepared microfluidic device is rinsed with relevant reagents to activate the microchannel network that allows proteins to attach. Then a series of operations such as capture antibody coating are carried out to obtain a microchip that can be used for the development of microfluidic ELISA.
In this step, we perform a series of manipulations in the prepared ELISA device (some microfluidic devices involve electricity or magnetism) to develop an ELISA format that meets your needs.
Analyze detected data and optimize where improvements can be made.
After optimizing the microfluidic ELISA development protocol, we will also test the biological samples you specify to ensure that the detection target of the protocol is consistent with your target to be tested.
After completing development, we will contact you to produce your specified microfluidic ELISA kit, including the microfluidic device and all reagents, with your consent.
Result/Material Delivery
Development Protocol
All Experi-mental Data
Ready-to-use ELISA Kits
Validated Assay Results
Creative Diagnostics provides you with microfluidic development services to meet your innovative needs for routine ELISA. In addition, we also provide corresponding ELISA testing services and provide comprehensive solutions for your customized ELISA kit needs. If you are interested in the development of microfluidic ELISA, please contact us directly.
Reference
- Lee, B., et al. (2009). "A fully automated immunoassay from whole blood on a disc." Lab on a chip.9(11), 1548–1555.
