Endocrinology
Application of Enzyme-Linked Immunosorbent Assay (ELISA) in Endocrinology Research
The endocrine system refers to the body fluid regulation system composed of all the endocrine glands and hormones in the body, and it is closely related to the central nervous system. Its main function is to release hormones under the control of the nervous system and based on material metabolism feedback, regulate the growth, development, reproduction, metabolism, movement, sickness, aging and other life phenomena of the human body, and maintain the relative stability of the human body environment. Symptoms that occur when the secretory function and/or structure of the endocrine glands or endocrine tissues themselves are abnormal are called endocrine diseases. It also includes the symptoms of abnormal hormone sources, abnormal hormone receptors, and physiological disorders caused by abnormal hormone or substance metabolism. Common endocrine diseases include hyperthyroidism/hypothyroidism, Cushing’s syndrome, and hypopituitarism. The endocrine examination is actually to see whether the secretion of these hormones is normal. Therefore, as an immunoassay method, ELISA is widely used in research on the endocrine system.
Common Targets in Endocrinology Research
Allopregnanolone
Adrenomedullin 2
Activin A Receptor Type I
17-Hydroxyprogesterone
Calcitonin
C-Peptide
Cholecystokinin 8
Dual Oxidase 2
Farnesoid X Receptor
Growth Hormone 2
Glucose Transporter 4
High Density Lipoprotein
Advantages of ELISA in Endocrinology Research
As a quick and convenient detection method, ELISA has the following advantages
- Can specifically detect the level of hormones in the sample
- Can help with the research of endocrine diseases
- Lower requirements for experimental equipment
- Convenient for researchers to monitor hormone levels
Common Targets Detected by ELISA in Endocrinology Research
Thyroid Stimulating Hormone
Thyroid-stimulating hormone (TSH) is one of the hormones secreted by the anterior pituitary gland. Its main function is to regulate the proliferation of thyroid cells, thyroid blood supply, and the synthesis and secretion of thyroid hormones. Diseases of the pituitary gland, thyroid, and hypothalamus can directly affect the synthesis and release of TSH. Determination of TSH in serum is one of the important indicators for the diagnosis and treatment of hyperthyroidism and hypothyroidism, as well as the study of the hypothalamic-pituitary-thyroid axis.
Insulin
Insulin is a protein hormone secreted by the islet β cells in the pancreas under the stimulation of glucose, lactose, glucagon, etc. The main function of insulin is to regulate the metabolic process. Insulin is the only hormone in the body that lowers blood sugar, and at the same time promotes glycogen, fat, and protein synthesis. Secondary diabetes caused by hyperaldosteronism, primary hypoparathyroidism, etc., and hypoglycemia caused by pancreatic islet B-cell tumors, hypoadrenalism function, and hypophyseal function, can all cause plasma insulin concentration reduction.
Adrenocorticotropic Hormone
Adrenocorticotropic hormone (ACTH) is a polypeptide hormone secreted by the pituitary gland of vertebrates. It can stimulate the development of the adrenal cortex and the synthesis and secretion of corticosteroids. ACTH detection can be used for the differential diagnosis of hyperfunction or hypofunction of the pituitary-adrenal cortex. Increased ACTH is common in stress states, primary adrenal insufficiency, and Cushing’s syndrome. ACTH reduction is common in hypopituitary gland function and damage to the anterior pituitary gland.
Creative Diagnostics has been committed to the application of ELISA in the field of endocrinology. Relying on strong R&D capabilities and advanced technology, we produce a variety of ELISA kits products for the research of endocrine, and we provide high-quality customized ELISA kits services, believable ELISA testing services, and professional ELISA development services related to endocrinology research according to your needs. If you want more information, please be at liberty to contact us.
References
- Szkudlinski, M.W.; et al. Thyroid-stimulating hormone and thyroid-stimulating hormone receptor structure-function relationships. Physiol Rev. 2002, 82(2): 473-502.
- Niswender, K.D. Basal insulin: physiology, pharmacology, and clinical implications. Postgrad Med. 2011, 123(4): 17-26.
