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Mercury Contaminants

The Severity of Environmental Contamination Caused by Mercury

Mercury (Hg) is widely used in industrial production. Mercury contaminants come from wastewater discharged from industries such as Chlor-alkali, plastics, batteries, electronics, and waste medical equipment. Mercury is one of the most toxic heavy metal elements in the environment, and the most dangerous organic compound of mercury is dimethyl mercury. The "Minamata disease" that first appeared in Japan in 1953 aroused worldwide concern about mercury poisoning. It is estimated that from 1970 to 1979, due to human activities in the world, about 16,000 tons, 100,000 tons, and 100,000 tons of mercury were directly discharged into water bodies, atmosphere, and soil, and the mercury discharged into the atmosphere and soil will also return to water bodies along with the water cycle. Methylmercury accumulates through the food chain, thereby endangering human health.

The Necessity of Monitoring Mercury Contamination with ELISA Testing

Mercury poisoning is a kind of neurotoxicity that can cause systemic nerve damage. The clinical manifestations of chronic metal mercury poisoning are mainly neurological symptoms, such as headache, dizziness, numbness of the limbs and movement disorders. Acute mercury poisoning occurs when a large amount of mercury vapor is inhaled, and its symptoms are hepatitis, nephritis, proteinuria, hematuria, and uremia. The International Agency for Research on Cancer of the World Health Organization has listed mercury and inorganic mercury compounds as three types of carcinogens, and mercury and mercury compounds have been listed as toxic and harmful water pollutants. In view of the strong toxicity of mercury, mercury contaminants in the environment should be strictly monitored. As a high sensitivity, high specificity, high accuracy, rapid and simple detection method, ELISA is widely used in the detection of mercury.

ELISA Type

Indirect Competitive ELISA

The Advantages of ELISA Testing

Price competitiveness
Portable enough to be used in the field
High-throughput detection and analysis of mercury
Simple operation without complicated equipment

ELISA Procedure for Heavy Metal Ions Mercury (II) Testing

The microplates were coated with CH₃Hg-MNA-OVA conjugate (200 μL/well) in carbonate buffer, pH 9.8, and incubated overnight at 4°C.

After washing three times with PBST, then blocked with blocking buffer (280 μL/well) for 1 h at room temperature.

After washing, Hg²⁺ standard solutions or samples (100 μL/well) and diluted mAb (100 μL/well) were added and incubated for 1 h at room temperature.

After washing, HRP-GaMIgG was added (200 μL/well) and the plate was incubated for 1 h at room temperature.

Then, the washed plate was added with the substrate solution (200 μL/well), incubated with shaking for about 20 min.

Terminated by adding sulfuric acid (5%, 80 μL/well) and the absorbance measured at 450 nm using microplate reader.

Creative Diagnostics has been committed to heavy metal contaminants testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of mercury. If you wish a lot of careful data, please contact us.

References

Nguyen, H.T.; et al. The influence oflong-range transport on atmospheric mercury on Jeju Island, Korea. Sci Total Environ. 2010, 408(6): 1295–1307.
Falandysz, J.; et al. Environmental cycling and fate of mercury: 2020. Chemosphere. 2020, 261: 127766.
Motts, J.A.; et al. Novel biomarkers of mercury-induced autoimmune dysfunction: a cross-sectional study in Amazonian Brazil. Environ Res. 2014, 132: 12-18.
Wang, Y.; et al. Highly sensitive and specific determination of mercury (II) ion in water, food and cosmetic samples with an ELISA based on a novel monoclonal antibody. Anal Bioanal Chem. 2012, 403(9): 2519-2528.

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