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The Severity of Environmental Contamination Caused by Organophosphorus Pesticides

Fig. 1 The chemical structure of chlorpyrifos

Organophosphorus pesticides (OPs) refer to organic compound pesticides containing phosphorus, which are mainly used to prevent and control plant diseases, insects, and weeds. Most Chlorpyrifos pesticides are divided into three categories: high toxicity, poisoning, and low toxicity. The common ones are parathion, systemic phosphorus, phorate, methyl parathion, methyl systemic phosphorus, malathion, and dibromophos, etc. The extensive use of OPs has caused them to enter water bodies, atmosphere, and soil widely, pollute the environment, and pose a threat to human health. Some types of OPs are not easy to oxidize, decompose, and are difficult to be degraded by microorganisms. They can stay in the environment for a long time and may undergo chemical changes to become more toxic and harmful secondary pollutants.

The Necessity of Monitoring Organophosphorus Pesticide Residues with ELISA Testing

Organophosphorus Pesticides Testing

Organophosphorus pesticides can enter the human body through the digestive tract, respiratory tract, and intact skin and mucous membranes. It forms phosphorylated cholinesterase with cholinesterase in the body, inhibits the decomposition of acetylcholine, leads to excessive acetylcholine in the tissues, overexcites cholinergic nerves, and causes muscarinic, nicotinic, and central nervous system symptoms. In severe cases, death can be caused by pulmonary edema, cerebral edema, and respiratory paralysis. With the increasing public concern about pesticide pollution in the environment, pesticide residues testing has more extensive and stricter requirements. In order to detect whether organophosphorus pesticides remain in surface water and their residual levels, ELISA has become an important detection method for the determination of pesticide residues in the past few decades.

ELISA Type

Competitive Indirect ELISA

The Advantages of ELISA Testing

High sensitivity and specificity to OPs
Can detect OPs at low cost and quickly
High-throughput detection and analysis of OPs

ELISA Procedure for Organophosphorus Pesticide Residues Testing

The microtiter plates were coated with hapten-OVA in carbonate-bicarbonate buffer (50mM, pH 9.6), and incubated overnight at 4°C.

After washing, the microtiter plates were blocked with 1% gelatin in PBS (200 μL/well), incubated for 1 h at room temperature.

50 μL/well of analyte dissolved in 10% MeOH-PBS (10mM, pH 7.4) and 50 μL/well of the ascites diluted with PBS (90mM, pH 7.4) were added to the plates and incubated for 1 h at room temperature.

After washing,100 μL/well of diluted (1/3000) goat antimouse IgG-HRP was added and incubated for 1 h at room temperature.

Followed by the addition of 100 μL/well of a TMB solution (400 μL of 0.6% TMB-DMSO and 100 μL of 1% H₂O₂ diluted with 25 mL of citrate-acetate buffer at pH 5.5), incubated for an appropriate time.

Terminated by adding 50 μL of 2M H₂SO₄, and the absorbance was read at 450 nm using a microplate reader.

Creative Diagnostics has been committed to pesticide residues testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of OPs. If you wish a lot of careful data, please contact us.

References

Piao, Y.Z.; et al. Development of ELISAs for the class-specific determination of organophosphorus pesticides. J Agric Food Chem. 2009, 57(21): 10004-10013.
Tang, J.; et al. Development of IC-ELISA for detection of organophosphorus pesticides in water. J Environ Sci Health B. 2008, 43(8): 707-712.

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