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Pyrethroid Pesticides

The Severity of Environmental Contamination Caused by Pyrethroid Pesticides

Pyrethroid Pesticides Testing Fig. 1 The chemical structure of deltamethrin

Pyrethroids are a class of biomimetic pesticides synthesized by structural modification of natural pyrethrins and are widely used in the prevention and control of various crop diseases and insect pests. Such pesticides include cypermethrin, lambda-cyhalothrin, rich-d-trans allethrin, etc. Pyrethroid contaminants mainly come from extensive use in agricultural production. In addition, the waste gas, wastewater, and waste discharged by enterprises that produce and process such pesticides are also the source of the contaminant. After a large amount of pyrethroid vinegar enters the environment, it is easy to accumulate in the environment for a long time and is leached and penetrated sedimentary rocks and groundwater through rainwater, causing water and soil pollution. Studies have shown that pyrethroids not only accumulate, but long-term exposure can also cause many chronic diseases in the human body.

The Necessity of Monitoring Pyrethroid Pesticide Residues with ELISA Testing

Pyrethroid Pesticides Testing

Pyrethroids are fat-soluble pesticides, which can not only accumulate in crops, even endanger animal and human health through the food chain. Acute poisoning symptoms are mainly manifested as neurological symptoms and skin symptoms. Nervous system symptoms include short-term unresponsiveness, fatigue, dyskinesia, palpitations, and then general excitement and convulsions. Skin symptoms are mostly localized allergies, red rashes, and tingling sensation around the eyes, nose, mouth, and contact parts. Pyrethroids enter the human body through the food chain and accumulate in the human body, causing carcinogenic, squirting, mutagenic, and other hazards. To prevent pyrethroids from continuing to pollute the environment and endanger biological health, ELISA can be used to detect the concentration of pyrethroids in environmental samples, and timely monitoring of such contaminants.

ELISA Type

Indirect Competitive ELISA

The Advantages of ELISA Testing

Capable of high-throughput detection of pyrethroids pesticide residues
Can detect pyrethroids quickly and at a low cost
Detection and analysis of pyrethroids without extensive pretreatment

ELISA Procedure for Deltamethrin Pesticide Residues Testing

The microtiter plates were coated with the antigen C134-AD (0.45 μg/mL in coating buffer, 100 μL/well), and incubated overnight at 4°C.

After washing four times with PBST, 50 μL/well of the deltamethrin standards or samples were added, followed by the solution of antisera As360, and incubated 30 min at RT.

After washing four times with PBST, a solution of anti-IgG-HRP (1/6000 in PBST) was added to the wells (100 μL/well) and incubated for 30 min at RT.

After washing four times with PBST, the substrate solution was added (100 μL/well) and incubated for 30 min at RT.

Terminated by adding 50 μL of 4N H₂SO₄, and the absorbance was read at 450 nm by a microplate reader.

Creative Diagnostics has been committed to pesticide residues testing by ELISA. Supported by rich professional knowledge and diversified ELISA kits products, we provide high-quality customized ELISA kits services, professional ELISA testing services, and ELISA development services, related to the detection of pyrethroid. If you wish a lot of careful data, please contact us.

References

Wolansky, M.J.; Harrill, J.A. Neurobehavioral toxicology of pyrethroid insecticides in adult animals: a critical review. Neurotoxicol Teratol. 2008, 30(2): 55-78.
Moschet, C.; et al. Picogram per liter detections of pyrethroids and organophosphates in surface waters using passive sampling. Water Res. 2014, 66: 411-422.
Fruhmann, P.; et al. Immunoassay and amperometric biosensor approaches for the detection of deltamethrin in seawater. Anal Bioanal Chem. 2018, 410(23): 5923-5930.

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