Products

Quinolones Residues

The Harm Caused by Food with Quinolones Residues

The Harm Caused by Food with Quinolones Residues Fig. 1 The chemical structure of Ciprofloxacin

Quinolones, also known as pyridone acids, are chemically synthesized antibacterial drugs that mainly act on gram-negative bacteria, Pseudomonas aeruginosa, mycoplasma, and chlamydia. Quinolone drugs are divided into four generations, and the third generation is currently clinically used. Commonly used drugs include norfloxacin, ofloxacin, ciprofloxacin, fleroxacin, and so on. Quinolone drugs inhibit bacterial replication by inhibiting bacterial desoxyribonucleic acid gyrase. With the widespread application of quinolones, they often remain in animal foods. Bacterial resistance and adverse reactions have also occurred one after another. After human consumption of animal food containing quinolone residues, the human body will become severely resistant to the drug. Long-term consumption of food contaminated by quinolones may cause serious adverse reactions. Mainly include insomnia, dizziness, headache, and other central nervous system symptoms; arthropathy; skin and phototoxicity; nausea, vomiting, diarrhea, and other digestive tract reactions; liver and kidney function abnormalities; blood cells and thrombocytopenia, hemolytic anemia, and other blood system symptoms.

The Necessity of Monitoring the Residue of Quinolones in Food by ELISA

Quinolones are widely used in animal husbandry and aquaculture because of their wide antibacterial spectrum, strong antibacterial activity, and no cross-resistance with other antibacterial drugs. This has also led to quinolones residues in most animal-derived foods. The problem of quinolones residues has attracted more and more attention. The Food and Agriculture Organization of the United Nations, the World Health Organization, and the European Union have all established maximum residue limits for a variety of quinolones in animal tissues. The US FDA announced in 2005 that enrofloxacin was banned from being used to treat bacterial infections in poultry. Quinolone broad-spectrum antibacterial drugs are mainly used for the treatment of human diseases. In order to prevent humans from consuming food containing quinolones residues and causing harm to the human body, it is necessary to detect such drugs in food. Currently, the commonly used detection method is enzyme-linked immunosorbent assay.

ELISA Types

Indirect competitive ELISA

The Advantages of ELISA Testing

Can monitor food safety and contribute to human health
Can quantitatively analysis of quinolones residues in food
Can avoid the emergence of resistant bacteria to quinolones

ELISA Procedure for Quinolones Residues Testing

The microplates were added with 100 μL/well of 0.1 μg/mL NOR₀–BSA that was diluted in 0.01M CB, incubated at 37℃ for 2 h.

After washing thrice with 10 mmol/L PBST, the 300 µL/well of 0.01M CB containing 0.2% (w/v) gelatin was added, incubated at 37℃ for 2 h.

After washing, 50 μL of each hapten in different dilutions and 50 μL of 0.1 μg/mL mAb were added and incubated at 37℃ for 30 min.

After washing thrice, 100 μL/well of HRP-conjugated goat anti-mouse IgG (diluted to 1:3000) was added, incubated at 37℃ for 30 min.

After washing four times, 100 μL/well of TMB solution (2 mL of 0.06%, w/v, TMB in glycol with 10 mL of 0.1M citrate phosphate buffer, pH 5, containing 1.8 μL of 30% hydrogen peroxide) was added and incubated at 37℃ for 30 min.

Terminated by the addition of 50 μL/well 2M H₂SO₄, the absorbance was measured at 450 nm by a microplate reader.

Creative Diagnostics has been committed to veterinary drug residues testing by ELISA. We provide reliable ELISA kits for the detection of quinolones. Supported by rich professional knowledge, we provide high-quality customized ELISA kits services, professional ELISA testing services, and believable ELISA development services related to quinolones residues. If you wish a lot of careful data, please contact us.

Reference

Zhen, Wang.; et al. Using hapten cross-reactivity to screen heterologous competitive antigens for improving the sensitivity of ELISA. Food Chem. 2020, 303: 125379.

Online Inquiry

Navigation