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Sulfonamides Contaminants

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The Severity of Sulfonamides Contamination

In a 2002 report, the U.S. Geological Survey announced 95 new contaminants in American waterways. Among them, the problem of sulfonamides contaminants is particularly prominent. Sulfonamides are a class of synthetic antibacterial drugs, which have killing effects on most gram-negative bacteria and gram-positive bacteria, so they are widely used. Although these drugs and metabolic intermediates can be partially removed through adsorption, biological and abiotic degradation in the environment, these drugs are difficult to be completely degraded in the environment, and will eventually enter environmental water bodies, threatening the safety of aquatic organisms and even humans. Even low concentrations of antibiotics may also lead to the emergence of drug resistance, and sulfa drugs in the environment may cause the emergence of drug-resistant bacteria and bring about biological safety problems.

The Necessity of Monitoring Sulfonamides Contamination with ELISA Testing

Fig. 1 The chemical structure of sulfa drugs

Sulfonamides can be used for prevention and treatment as veterinary drugs, and can also be added to feed as growth promoters. At the same time, sulfa drugs are commonly used therapeutic drugs for humans, mainly for the treatment of bacterial infections. It is estimated that they account for 16-21% of annual antibiotic usage. These compounds are excreted into the environment and become environmental contaminants that endanger biological health. In order to reduce pharmaceutical contaminants in the environment, ELISA testing is used to monitor the concentration and source of these drugs.

ELISA Type

Direct ELISA

The Advantages of ELISA Testing

Easy to use
Lower cost
High-throughput detection and analysis
High sensitivity and specificity

ELISA Procedure for Sulfonamides Contaminants Testing

75 µL/well of standards (0, 0.025, 0.05, 0.1, 0.25, and 1 µg/L) in water or samples were added to the plate.

Followed by the addition of horseradish peroxidase (HRP) (50 µL/well) and further incubated for 40 min at room temperature.

A solution of hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine (TMB) (150 µL/well) was added to the plate and incubated at room temperature for 30 min.

Then add 50 µL/well of primary antibody and incubate at room temperature for 20 min.

Plates were washed with 250 µL/well of phosphate-buffered saline containing 0.05% Tween 20 (PBST) three times.

Terminated by the addition of 100 µL/well of 1M H₂SO₄, the absorbance was read at 450 nm.

Creative Diagnostics has been committed to pharmaceuticals contaminants testing by ELISA. We provide ELISA kits products, customized ELISA kits services, ELISA testing services and ELISA development services related to the detection of sulfonamides contaminants. If you wish a lot of careful data, please be at liberty to contact us.

References

Göbel, Aa.; et al. Extraction and determination of sulfonamides, macrolides, and trimethoprim in sewage sludge. J Chromatogr A. 2005,1085(2).
Shelver, W.L.; et al. ELISA for sulfonamides and its application for screening in water contamination. J Agric Food Chem. 2008,56(15): 6609-6615.

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