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Tetracyclines Residues

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The Harm Caused by Food with Tetracyclines Residues

The Harm Caused by Food with Tetracyclines Residues Fig. 1 The chemical structure of Tetracycline

Tetracyclines are a class of broad-spectrum antibiotics produced by actinomycetes. Tetracyclines have a killing effect on gram-positive bacteria, gram-negative bacteria, anaerobic bacteria, most rickettsia, mycoplasma, chlamydia, atypical mycobacteria, and spirochetes. Tetracyclines antibiotics mainly include chlortetracycline, oxytetracycline, tetracycline, and semi-synthetic derivatives of methenoxycycline and doxycycline. Tetracyclines can specifically bind to the A position of the 30S subunit of the bacterial ribosome to prevent the connection of aminoacyl-tRNA at this position, thereby inhibiting the growth of peptide chains and affecting the synthesis of bacterial proteins. The widespread use of tetracyclines has led to their frequent residues in animal foods. Long-term consumption of food contaminated with tetracycline may cause serious adverse reactions. Mainly manifested as vomiting, abdominal pain, diarrhea, esophageal ulcers, and other gastrointestinal reactions; multiple bacterial infections; effects on bone and tooth growth; liver damage; vitamin deficiency; nephrotoxicity; in addition, tetracycline antibiotics can occasionally cause drug fever, skin rashes, and other allergic reactions.

The Necessity of Monitoring the Residue of Tetracyclines in Food by ELISA

Tetracyclines as broad-spectrum antibacterial veterinary drugs have a wide range of applications in aquaculture, livestock breeding, and other industries. In addition, tetracyclines will also be used as feed and fertilizer additives to increase the antibacterial properties of animals and plants. However, improper use or non-compliance with the limit regulations will cause residues in livestock products and cause serious harm to human health. Therefore, feed testing and fertilizer testing will involve the limited analysis of tetracyclines. The requirements for residue limits of tetracyclines in different foods and commodities are different. However, the detection standards for tetracyclines residues have clear regulations on their limit indicators, detection methods, and quantitative analysis methods. The ELISA with convenient operation, simple process, and high accuracy is often used to quantitatively analyze the tetracyclines residues in food.

ELISA Types

Indirect competitive ELISA

The Advantages of ELISA Testing

Can prevent food contaminated by tetracyclines from entering the market
Can quickly and quantitatively analyze the tetracyclines residues in food
Can help the development of the food industry and benefit human health

ELISA Procedure for Tetracycline Residues Testing

The microplates were coated with 100 μL/well of appropriate coating conjugate solution in CB, incubation at 4℃ overnight.

After plates were washed six times with 10 mmol/L PBS-T, the 50 μL of the serum dilution and 50 μL of TCs in PBS-T were added and incubated at room temperature for 1 h.

After washing again as the previous step, 100 μL/well of GAR-HRP (diluted 1/4000 in PBS-T) was added, incubated at room temperature for 1 h.

After washing, 100 μL/well of substrate solution (2 mg/mL OPD and 0.012% H₂O₂ in 25 mmol/L sodium citrate and 62 mmol/L sodium phosphate, pH 5.5) was added and kept for 10 min.

Terminated by the addition of 50 μL/well 2.5 mol/L H₂SO₄, the absorbance was measured at 450 nm by a microplate reader.

Creative Diagnostics has been committed to veterinary drug residues testing by ELISA. We provide reliable ELISA kits for the detection of tetracyclines. Supported by rich professional knowledge, we provide high-quality customized ELISA kits services, professional ELISA testing services, and believable ELISA development services related to tetracyclines residues. If you wish a lot of careful data, please contact us.

References

Chopra, I.; Roberts, M. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol Rev. 2001, 65(2): 232-260.
Pastor-Navarro, N.; et al. Synthesis of haptens and development of a sensitive immunoassay for tetracycline residues. Application to honey samples. Anal Chim Acta. 2007, 594(2): 211-218.

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